A regular nanostructured dithiolene metal complex film for ultrasensitive biosensing of liver enzyme

نویسندگان

  • Danfeng Jiang
  • Cuiping Li
  • Tao Liu
  • Linlin Li
  • Zhenyu Chu
  • Wanqin Jin
  • Xiaoming Ren
چکیده

A novel ultrasensitive biosensor was constructed by the [TBA]2[Ni(mnt)2]/graphene (mnt = maleonitriledithiolate) composite film for the detections of glutamate and alanine aminotransferase (ALT). The [TBA]2[Ni(mnt)2] nanocubes with a dimension of 500 nm were in-situ crystallized on the surface of Au electrode modified using thiol graphene in advance. Due to the synergistic effect

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

MOLECULAR WEIGHT DETERMINATION AND METAL ION REQUIREMENT OF PHOSPHATIDATE PHOSPHOHYDROLASE PURIFIED FROM CYTOSOLIC FRACTION OF RAT LIVER

Phosphatidate phosphohydrolase (PAP) from cytosolic fraction of rat liver was purified to homogeneity having specific activity of 5.14 U/mg protein. An activity staining procedure was developed to determine molecular weight of the enzyme on polyacrylamide gel electrophoresis using Ferguson plot. Molecular Weight (M.W.) of the active PAP was 298 KDa. SDS-PAGE analysis showed a M.W. of 47 KDa for...

متن کامل

Bis-three-way junction nanostructure and DNA machineries for ultrasensitive and specific detection of BCR/ABL fusion gene by chemiluminescence imaging

A novel G-quadruplex DNAzyme-driven chemiluminescence (CL) imaging method has been developed for ultrasensitive and specific detection of BCR/ABL fusion gene based on bis-three-way junction (bis-3WJ) nanostructure and cascade DNA machineries. Bis-3WJ probes are designed logically to recognize BCR/ABL fusion gene, which forms the stable bis-3WJ nanostructure for the activation of polymerase/nick...

متن کامل

Detection of trace heavy metal ions in water by nanostructured porous Si biosensors.

A generic biosensing platform, based on nanostructured porous Si (PSi), Fabry-Pérot thin films, for label-free monitoring of heavy metal ions in aqueous solutions by enzymatic activity inhibition, is described. First, we show a general detection assay by immobilizing horseradish peroxidase (HRP) within the oxidized PSi nanostructure and monitor its catalytic activity in real time by reflective ...

متن کامل

In-situ fabrication of well-distributed gold nanocubes on thiol graphene as a third-generation biosensor for ultrasensitive glucose detection

Because of the advanced work mechanism on enzyme direct electron transfer, third-generation biosensor has aroused the growing attentions in recent decade. Herein, a novel structured 3rd generation glucose biosensor was constructed by the in-situ limited electrodeposition of gold nanocubes on a thiol graphene film. On account of the restriction from –SH group, initially, a tightly distributed Au...

متن کامل

Recent progress in Prussian blue films: Methods used to control regular nanostructures for electrochemical biosensing applications.

In the last decade, Prussian blue (PB) has attracted increased scientific interest in various research fields, such as fuel cells, gas separation and pollution treatment. Due to its advanced catalysis, biocompatibility, selectivity and stability, PB has been widely used in biosensor construction. However, the formation of regular PB nanostructures is challenging due to its fast crystallization ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2016